Melatonin Enhances the Chemosensitivity of Pancreatic Carcinoma Cells (PANC-1) to Cisplatin and Cetuximab through modulation of p21, p27, p53, p57, MDM2 and KRAS Genes

We investigated the effects of melatonin, cetuximab and cisplatin treatments alone or in combination on PANC-1 cells from a human pancreatic carcinoma ductal cell origin through viability gene expressions. The were left for 48 h incubation after applying chemicals cells. metabolic substances at end measured by MTT assay. expressions p21, p27, p53, p57, MDM2 KRAS determined RT-PCR. use melatonin combined with increased p21 p57 genes decreased gene. Furthermore, p27 expression compared to alone. was lowest plus and/or groups. p53 highest groups while its autoregulator In conclusion, combinations enhances tumor suppressor along modulation oncogenic suggesting potential as therapeutic approach therapy adenocarcinoma.